Rol del xantano en la formación de biopelículas durante la vida epifítica de Xanthomonas citri subsp. citri y su relación con el desarrollo del cancro
Palavras-chave:
Citrus limon, exopolisacáridos, cancrosis, microscopía confocal de barrido láser, gen gumB, exopolysaccharides, citrus canker, confocal laser scanning microscopy, gumB geneResumo
La mayoría de las bacterias fitopatógenas viven de forma epifítica sobre las plantas antes de la colonización y sobreviven en la superficie del tejido del hospedante, mediante la formación de biopelículas. La capacidad para producir exopolisacáridos es crítica para la formación de las mismas en diversas bacterias. En este trabajo, se estudió la formación de biopelículas de X. citri subsp. citri, bacteria responsable de la cancrosis de los cítricos, y su relación con la supervivencia epifítica y el desarrollo del cancro sobre hojas de plantas de limonero. Mediante tinción con cristal violeta y análisis de microscopía confocal de barrido láser, se observó que X. citri subsp. citri forma estructuras de biopelículas sobre hojas de limonero. En contraste, una cepa mutante defectiva en la biosíntesis del exopolisacárido xantano, denominada X. citri subsp. citri gumB, fue incapaz de formar dichas estructuras y su supervivencia epifítica y capacidad de desarrollar la enfermedad en la planta se vieron comprometidas. Estos resultados sugieren que la formación de biopelículas tiene una importancia clave en la fase epifítica de X. citri subsp. citri antes y durante el desarrollo del cancro.
ABSTRACT
Role of xanthan in biofilm formation during Xanthomonas citri subsp. citri epiphytic life and its relationship to canker development
Almost all phytopathogenic bacteria have an epiphytic life before invading the host plant. These bacteria survive on the surface of the host tissue through biofilm formation. The ability to produce exopolysaccharides is critical for biofilm formation in several phytopathogenic bacteria. In this work, biofilm formation by Xanthomonas citri subsp. citri (bacterium responsible for citrus canker disease), and its relationship to epiphytic survival and canker development on lemon leaves were studied. Through crystal violet staining and confocal laser scanning microscopy analysis, it was observed that X. citri subsp. citri forms biofilm structures on lemon leaves. Furthermore, a X. citri subsp. citri gumB mutant strain, defective in production of exopolysaccharide xanthan, did not form a structured biofilm and showed reduced growth and survival on leaf surfaces, and reduced disease symptoms. These results suggest that biofilm formation has an important role in X. citri subsp. citri epiphytic survival prior to canker development.
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